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KMID : 0616120210520030163
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Korean Journal of Pharmacognosy
2021 Volume.52 No. 3 p.163 ~ p.169
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Antioxidant Activity of Ethyl acetate Fraction of the Guzeunggupo-procossed Platycodon grandiflorum A. De Candolle roots in Caenorhabditis elegans
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Kwon Kang-Mu
Kim Jun-Hyeong
Yang Jae-Heon
Ki Byeol-Hui
Hwang In-Hyun
Kim Dae-Keun
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Abstract
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Using the Caenorhabditis elegans model system, the antioxidant activity of methanol extract of the guzeunggupo-processed Liriope platyphylla F. T. Wang (Liliaceae) tuber was calculated. Between the methanol extracts of guzeunggupo-processed and non-processed L. platyphylla tuber, the processed L. platyphylla tuber showed higher DPPH radical scavenging effect than the non-processed one. The ethyl acetate soluble fraction of the methanol extract of the guzeunggupo-processed L. platyphylla tuber showed the best DPPH radical scavenging activity. The ethyl acetate fraction of the processed sample was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species level. In addition, to verify the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction of the processed sample, SOD-3 expression was measured using a transgenic strain (CF1553). Consequently, the ethyl acetate fraction of the processed sample, increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. Furthermore, the ethyl acetate fraction of the processed sample-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.
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KEYWORD
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Guzeugupo-processed Liriope platyphylla, Caenorhabditis elegans, Antioxidant activity, SOD, Catalase
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